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Quantitative DNA method in forensic DNA laboratory

 

Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
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  • Product Description
 

Quantification of human DNA in forensic samples is an important step during STR profiling because the STR genotyping is sensitive to the quantity of DNA used in the PCR reaction.This study focuses on the importance of quantification in the entire process of genetic analysis.Two real time PCR platforms were used to compare two commercial kits in terms of DNA quantification.It was found out that accuracy of absolute quantification values in commercial quantification kits is strongly dependent on the construction of calibration curve. Especially low template DNA samples were used to assess whether QuantifilerTM or Plexor® HY System can determinate a minimum quantification value(cut off value)below which STR profiles would consistently fail to be detected. The usage of Plexor® HY System enabled to determine the cut off quantification value more exactly probably due to different molecular background and chemistry used in this kit.Reliability and other issues connected with cut off value are discussed. In order to better understand the relationship between the quantity of DNA and the number of detectable loci series the dilution expexperiment with standard DNA007 was done.

Product Specifications
SKU :COC88540
AuthorIvana Koljensic
LanguageEnglish
BindingPaperback
Number of Pages88
Publishing Year2013-12-12T00:00:00.000
ISBN9783659415807
Edition1 st
Book TypeGenetics (non-medical)
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-10-08 00:00:00
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