Plant growth promoting rhozobacteria (PGPR) are known to influence plant growth by various direct or indirect mechanisms. Environmental protection and the need to enhance agricultural output have made research in new sustainable technologies necessary. As in most sectors of agriculture, there is increasing demand for organic tomatoes. Therefore, the overall strategy for increasing crop yields, sustaining them at a high level and maintaining proper soil health must include an integrate approach to develop effective and more consistent active plant growyh promoting rhozobacteria (PGPR) inocula, because, there is a considerable experimental support for the idea that PGPR may be used as biofertilizers and biocontrol agent to increase the early growth of seedlings in the nursery and subsequently the yield with concomitant decrease in the chemical fertilizers and pesticides. Therefore, the aim of this study is to determine the effect of inoculation with PGPR’s on growth of tomato seedlings in growth chamber and in green house. The bacterial strains were characterized for antifungal-antibiotic activity and plant growth regulators under in vitro conditions.