Sunflower is an important oilseed cash crop in the world. Several biotic and abiotic stresses reduced productivity of sunflower. Among these root rot caused by Macrophomina phaseolina is an important disease through out the world. The disease may be managed by resistant varieties, chemical nematicides and biological control agents. Bioformulation is a biotechnological approach and is successfuly used in vitro and under field conditions.