The research was conducted in 2008 to study seroprevalence of brucellosis in buffalo, goat and pig in Nepal. Total 376 blood samples (5ml from each) were collected randomly from buffaloes, goats and pigs from different part of Nepal. They were kept in cold box and brought in laboratory to centrifugation for collecting serum. Thus obtained serum samples were tested by Brewer Diagnostic Card. Positive reactors showed up characteristic agglutination after wet incubation for 4 minutes. Out of 153 sera-samples of buffalo subjected to card test, all showed up to be negative reactors, while out of 70 sera-samples of goat and 153 sera-samples of pig subjected to card test, 12 (i.e. 17.14%) and 11 (i.e. 7.18%) showed up to be positive reactors respectively. The females showed high prevalence of 18.6% (11/59) than that of males of 9.1% (1/11) in goat. Similarly the females of pigs showed high prevalence of 9.23% (6/65) than that of males of 5.7% (5/88). The result obtained of sero-prevalence of brucellosis of different meat animals of Nepal showed that the prevalence rate is higher in goats than that of pigs and buffaloes.