Soil organic matter characterization in arable soils is essential to sustain agricultural lands. The present investigation was initiated with some representative soils of Khulna and Jessore district to characterize organic matter under different cropping patterns. The study has a scope to focus on the effects of organic matter characterization of four soil series under different cropping patterns of the study areas. Laboratory investigations were conducted in Soil science discipline, Khulna University to characterize organic matter of Ganges meander flood plain soils under different cropping patterns. Sixteen representative soil samples from different locations of Ganges meander flood plain soils were collected for study. The soils belong to Amjhupi, Ishurdi, Mirpur and Sara series. Organic matter characterization was done by humic acid, fulvic acid percentage, E4/E6 ratio, coagulation threshold value, and C/N ratio and they varied significantly under different cropping patterns.