In this study the effect of T.thiooxidans in the soil reclamation and sulphur solubilizing capabilities of Organism has been analyzed. The soil with different PH (7.5, 8.3 and 9.3) was tested with different concentration (20 ml, 30 ml and 50 ml) of T.thiooxidans. The soil was also treated with the combination of T.thiooxidans and sulphur particles in different concentrations (0.5%, 1% and 1.5%). From the above treatment, T.thiooxidans along with Sulphur particles was found to reduce the soil pH from alkaline to acidic in efficient manner at faster rate. The Sulphur oxidizing bacteria, T.thiooxidans was tested for Sulphur, Nitrogen, Phosphorus and Potassium production in soil. It was proved that increased concentration of T.thiooxidans resulted in increased production of Sulphur, Nitrogen, Phosphorus and Potassium in soil. In this study, the green gram plant was tested with different soil pH and different concentrations of T.thiooxidans and only with sulphur particles. The plant was checked for Protein, Chlorophyll and Carbohydrate content similar studies were made to check the length of shoot and root of the plant, the above tests proved that to be increased.