By the present investigation, it may be concluded that Karachi people have noticeable quantities of insecticides in their fat and blood. The blood samples have more pesticides than fat. The more quantity of dieldrin as compared to DDT indicates that DDT usage is now limited to some extent in Karachi as compared to twenty years early report of Mughal and Rehman (1973) showed heavily exposure of DDT in people living in this city. In contrast to this, people are now accumulating more dieldrin which may probably due to its greater usage in termite control programme. The reduction in ChE activity in pesticide exposed workers signals us that insecticides reduce the enzyme level by disturbing the normal biological functions of human. So in future, it is necessary to check the enzyme activity in pesticide preparing and manufacturing workers regularly and in general population yearly as the general population is also accumulating pesticides. Moreover it is also necessary to check total protein, ?- and ?-globulin, bilirubin, alanine and aspartate aminotransferases activity in pesticide handling people because any alteration in these measures may produce liver lesion and other disorders.