In recent years, the pet food industry has become a multimillion dollar business all around the world. There are several types of pet food available for cats and dogs that include dry foods, wet foods, canned foods, moist foods, semi-moist foods, frozen-chilled pet foods, and treats. Pet food generally consists of meat, meat byproducts, cereals, grain, vitamins, and minerals. this study investigate the effect of incorporation of tripe meal and rice flour on development, quality evaluation and storage stability of pet food under ambient condition. The quality of pet food was based on physicochemical characteristics namely moisture, ash, fat, protein content, pH, TBA number (Thiobarbituric acid) and microbial characteristic viz TPC (total plat count). The RSM (Response surface methodology) was applied to study the effect of tripe meal, rice flour and other ingredients and for optimization.