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Studies on FPA for the diagnosis of brucellosis in buffaloes


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  • Product Description

Brucellosis is a major bacterial zoonosis of global importance. The conventional serological tests viz. RBPT, SAT and CFT have certain inherent problems which are circumvented with the development of ELISA. But till date species specific conjugate required for ELISA in buffaloes is not available. In the present study, Fluorescence Polarization Assay was standardized using indigenously prepared antigen for the diagnosis of brucellosis in buffaloes. The OPS was extracted from B. abortus S99 and conjugated with FITC to obtain FPA antigen. The fluorescence intensity of the FPA antigen was adjusted between 2,50,000 and 3,00,000. The cutoff for FPA was determined to be 116.8mP units using ROC analysis program of MedCalc Software. The sensitivity, specificity and P.I. of FPA were 92.95%, 98.17% and 191.12, respectively and were comparable to those observed with anti-bovine ELISA and Protein-G ELISA. The sensitivity and P.I. values of FPA were much higher than SAT and RBPT, however specificity values of FPA, SAT and RPBT were comparable. The Kappa test revealed perfect agreement between FPA, anti-bovine ELISA and Protein-G ELISA and only a substantial agreement between FPA and RBPT.

Product Specifications
SKU :COC45750
AuthorManish Kumar and Puran Chand
Number of Pages160
Publishing Year2012-05-08T00:00:00.000
Edition1 st
Book TypeMicrobiology (non-medical)
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-06-08 00:00:00
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