Understanding the variations in the physico-chemical and microbiological aspects of the Palk Strait will help to understand the ecological functioning. Moreover, changes in physico-chemical properties of the prevailing environment will decide the composition of biological diversity; this is applicable from microbes to mammals. The occurrence of microbes in the environment will change spatially and temporally and hence understanding of the mercury-resistant bacteria become important and thereby helps to protecting the human population from water borne diseases. Having this in mind, the present was carried out. ? Totally, 47 strains were isolated based on colony morphology from SWNG plates for identification. Only four (BHRM) strains showed a higher resistance to mercury. The four strains identified were UG12: Bacillus cereus; UG18: Vibrio parahemolyticus; UG22: Escherichia coli and UG28: Pseudomonas aeruginosa.