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Study of retention mechanism and development of HILIC methods

Study of retention mechanism and development of HILIC methods

 

Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
Delivery in :  10-12 Business Days

 
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  • Product Description
 

In the last twenty years hydrophilic interaction liquid chromatography (HILIC) has emerged as a new type of liquid chromatography for separation of polar compounds. In HILIC, a multimodal retention mechanism is present so it is very difficult to predict the impact of chromatographic parameters on the retention and separation of the compounds. Furthermore, the relationship between the pH of the mobile phase, the degree of ionization of the compounds, the surface of the stationary phase and the absorbed water layer is very complex, which significantly complicates the HILIC method development. In this book we experimentally determinate the contribution of partition, adsorption and ion exchange mechanism to the retention of several polar compounds on bare silica column. Also fast, simple and easily applicable HILIC methods for determination of polar compounds in different dosage forms were established using the univariate and design of experiments approach. The book could serve as a guide for the HILIC method development and optimization, and should be useful for practitioners working in analytical method development laboratories.

Product Specifications
SKU :COC85234
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-10-08
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