A series of 1-(substituted phenyl)-3-(4-oxo-2-phenylquinazolin-3(4H)-yl) urea were synthesized and evaluated for their cytotoxic activity against human cervical cancer cell line (HeLa) and NIH 3T3 mouse embryonic fibroblasts cell line by MTT assay method. We found that compound 2d inhibited proliferation of human cervical cancer cell line (HeLa) IC50 6.96(µM) concentration. Which was more potent than the standard 5-flourouracil (8.46 µM). Other compound was found to be a less potent. 2d could be further utilized for the design of newer and more effective anticancer agents against for cervical cancer. Since the compounds were synthesized in a few steps, they may be apt to an eco friendly approach at a large scale level.