Cytokines play an important role in the pathology associated with chronic inflammatory diseases because of pro-inflammatory and neutrophil chemotactic properties. The biological effects of IL-8 are relevant in this regard. This study was done to compare the level of this molecule in Gingival Crevicular Fluid from patients with adult periodontitis and from individuals with clinically healthy gingival. GCF was collected from patients with adult periodontitis and clinically healthy gingival for 30 seconds using a periopaper strip and the volume of the sample determined. Following elution of the fluid, assays for IL-8 were carried out by ELISA. The results of the study showed that level of IL-8 of the experimental group were significantly higher than the control group (p>0.01). The clinical parameters were positively correlated to IL-8 suggesting that the GCF IL-8 exhibited dynamic changes upon severity of periodontal disease (p>0.05). Thus, to conclude, the level of IL-8 in GCF is valuable in detecting the inflammation of periodontal tissue.