Book describes the basic techniques that are used in molecular biology. These techniques includes various DNA manipulation techniques, cloning and bacterial growth. This book also describes the Green Fluorescent Gene, one of the novel achievements in the field of science. The reason for writing this book is that in our graduation and post graduation we heard about various molecular biology techniques but we know very little about these techniques. And even we know the techniques we are not much aware about the principle and the protocols of the techniques. I am writing this book for the graduate and post graduate students who want to know basic molecular techniques and also their protocols so that they can use it in their laboratory doing their work. Many researchers can also get help from this book.