The role of antioxidant activity of Thiourea was studied in VIGNA RADIATA plants were supplemented with UV B.This research compared the difference between UV B supplemented plants and Thiourea along with UV B supplemented plants. This study showed that UV B induced oxidative stress as evidenced by increased in lipidperoxidase, catalase, and super oxide dismutase content. The oxidative stress also showed by decreased in glutathione, starch, carbohydrate and chlorophyll content, when compared with control plant. Thiourea added plants showed modulations in UV B supplemented plants, in the amount of biochemical compounds and antioxidant enzyme system. Molecular Docking of thiourea with UV resistant protein showed positive interactions and this shows thiourea have a role in stabilizing oxidative homeostasis. Result of this research showed that thiouera can minimize the effect caused by UV B rays. The plant stress tolerance can be improved with the use of exogenous use of stress alleviating chemicals.