The study aimed at establishing gender dynamics of HIV and AIDS at university level of education in Kenya. Comprehensive data on the magnitude of HIV and AIDS was found to be lacking in all the universities. Where available, the data was not disaggregated by gender or category of respondents. Vulnerability to HIV and AIDS vary by gender, age, level of study, type of residence, economic group, category of respondents and geographical location. HIV and AIDS poses a threat to the human capital formation function of university education in Kenya, and ultimately the country’s socio-economic development. HIV and AIDS impacts both supply and demand of education, at individual, family/community, institutional and national levels. The availability, type and adequacy of the mitigatory measures/interventions varied by universities, are largely inadequate, not gender responsive and in most instances, not known to the target beneficiaries. The study recommends putting in place monitoring and evaluation frameworks to ensure appropraite documentation of data on HIV and AIDS, increased budgetary allocation and gender mainstreaming in institutional HIV and AIDS interventions.