In vitro propagation through callogenesis, organogenesis and total yield was studied and also observed the antimicrobial effect of Clitoria tenatea L. extracts. For callogenesis and organogenessis different combinations of auxin (2,4-D, NAA) and cytokinin (BAP, Kinetin) were examined. Methanolic extract of Clitoria ternatea L. seeds showed maximum zone of inhibition against bacterial stain (Azotobacter sp). The extracts of Clitoria ternatea L. (seeds, callus, in vitro and in vivo leaves) showed response against fungal strains Aspergillus niger, Alternalia solani and none of these extracts showed any response against Rhizopus oryzae. Against all bacterial and fungal strains the zone of inhibition of in vivo leaves extracts was more than in vitro leaves extracts.