The brain cells called neurons are meant to divide rapidly. Brain cancer starts in just one cell when a division starts dividing in an uncontrolled manner. As the cell makes more copies of itself, it grows to form a tumor, may be Benign or Malignant tumors. Immunohistochemical study of tissue Transglutaminase (tTG) in 22 brain tumor samples was performed. tTG expression was observed visually in endothelial and tumor cells. In most of the cases, the tTG expression was observed in endothelial cells but in few cases tTG expression was also observed in tumor cells along with endothelial cells. This shows that tumors were of high grade. We observed benign and malignant tumours like Meningioma, Papilloma, Adenoma, Astrocytoma, Glioblastoma, Multiforme Glioblastoma, Gemistocytic and Neurocytoma. Twelve females and ten males were among patients having age ranged from 8-61yrs.. The tumor were graded according to WHO grading system from grade one to grade four .Low grade tumors were more in comparison to high grade tumors. The tissue Transglutaminase expression was expressed in terms of percentage where high grade tumors showed higher percentage of expression compared to low grade ones.