Superficial fungal infections are very common infections, approximately 90% of fungal skin infections are caused by dermatophytes-types of fungi. The present status of topical antifungal therapeutics like creams, gels, ointments etc., suffer from many drawbacks like severe blistering, itching, redness, peeling, dryness, or irritation of the skin and failed to achieve mycological eradication. To overcome the aforementioned limitations associated with conventional formulations, the novel vesicular carrier systems i.e. (liposomes, ethosomes, transfersomes) were being used. These carrier systems was preferred for topical drug delivery as these are biodegradable, biocompatible and nontoxic carrier as they composed of natural lipid, that was compatible with intercellular lipid lamellae within the skin layer. They also act as penetration enhancers owing the penetration of the individual lipid components into the stratum corneum. Amphotericin B (AmB) is most effective drug for the treatment of Mycosis infections. Hence, AmB loaded vesicular carrier systems were prepared and studied their relative potential for topical delivery of AmB for the treatment of fungal skin infections.