VSV Pseudotypes Bearing HBsAg


Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
Delivery in :  10-12 Business Days

₹ 3,651

Availability: Out of stock


Delivery :

5% Cashback on all Orders paid using MobiKwik Wallet T&C

Free Krispy Kreme Voucher on all Orders paid using UltraCash Wallet T&C
Product Out of Stock Subscription

(Notify me when this product is back in stock)

  • Product Description

Analysis of hepatitis B virus (HBV) infection has been difficult due to a lack of suitable culture system. Pseudotyped viruses provide a safe viral entry model because of their inability to produce infectious progeny virus. Here a system has been developed to prepare vesicular stomatitis virus (VSV) pseudotypes bearing various surface proteins of HBV(HBsAg): S, M or L. The infectivities of the psedotypes were determined by quantifying the number of cells expressing green fluorescent protein (GFP). The infectious unit (IU) of the pseudotype samples neutralized with anti-VSV in the absence and presence of anti-HBs S MAbs and titrated on HepG2 cells ranged from 1,000 to 4,000 IU/ml and 200 to 400 IU/ml, respectively. VSV pseudotype sample bearing HBs M antigen showed the highest infectious titer. This infectivity was inhibited by treatment with lactoferrin or sulfated polysachharides. Pretreatment of the cells with trypsin, tunicamycin or DTT also inhibited the infectivity of the pseudotypes. The VSV?G*(HBV) pseudotypes might be useful as a safe and rapid assay system to clarify early steps of HBV infection.

Product Specifications
SKU :COC29369
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
0 Review(s)