Wildlife Law Enforcement plays a vital role in the conservation of biological diversity by ensuring that plant and animal species are protected. Although South Sudan has a Wildlife Act, it does not have a Wildlife policy to assist in the enforcement of existing laws. Hence, the country has witnessed an escalation in crime targeting biological resources that has led to decline and extinction of some species. This study was conducted in Badingilo National Park (BNP) in South Sudan with the following objectives: 1) identify the challenges facing wildlife law enforcement; 2) determine the causes and effects of the challenges faced in enforcing wildlife laws; and 3) come up with interventions to mitigate effects of challenges of wildlife law enforcement in BNP. Implementation of these interventions will enhance wildlife law enforcement and promote biodiversity conservation not only in BNP but also in other protected areas facing the same challenges in South Sudan.